Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formal Charges02:42

Formal Charges

40.7K
In some cases, there are seemingly more than one valid Lewis structures for molecules and polyatomic ions. The concept of formal charges can be used to help predict the most appropriate Lewis structure when more than one reasonable structure exists.
40.7K
Ions and Ionic Charges03:27

Ions and Ionic Charges

79.3K
In ordinary chemical reactions, the nucleus — which contains the protons and neutrons of each atom and thus identifies the element — remains unchanged. Electrons, however, can be added to atoms by transfer from other atoms, lost by transfer to other atoms, or shared with other atoms. The transfer and sharing of electrons among atoms govern the chemistry of the elements. During the formation of some compounds, atoms gain or lose electrons to form electrically charged particles called...
79.3K
Peptide Bonds02:43

Peptide Bonds

83.4K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
83.4K
Atomic Radii and Effective Nuclear Charge03:08

Atomic Radii and Effective Nuclear Charge

62.3K
The elements in groups of the periodic table exhibit similar chemical behavior. This similarity occurs because the members of a group have the same number and distribution of electrons in their valence shells.
62.3K
Electric Charges01:11

Electric Charges

23.1K
From lightning during thunderstorms to electronic devices, the phenomenon of electromagnetism is all around us. The electromagnetic force is one of the four fundamental forces of nature. It has been known to humanity in various forms for thousands of years. For example, the ancient Greek philosopher Thales of Miletus recorded his experiments on static electricity using amber and fur in the sixth century BC.
The English physicist William Gilbert studied the phenomenon of static electricity in...
23.1K
Charge on a Conductor01:26

Charge on a Conductor

5.4K
An interesting property of a conductor in static equilibrium is that extra charges on the conductor end up on its outer surface, regardless of where they originate. Consider a hollow metallic conductor with a uniform surface charge density. Since the conductor itself is in electrostatic equilibrium, there should not be any electric field inside the conductor. Now, assume a Gaussian surface enclosing the hollow portion. Applying Gauss's law, the inner surface of the hollow conductor will not...
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Storage Buffer Composition Impacts Internal Structure, Freeze-Thaw Stability, and Transfection Efficiency of mRNA-Lipid Nanoparticles.

ACS nano·2026
Same author

Mg<sup>2+</sup> Catalyzes Nonenzymatic RNA Primer Extension through a Concerted Outer-Sphere Mechanism.

Journal of the American Chemical Society·2026
Same author

Measuring Differences in Protein Allosteric Graphs Constructed via Molecular Dynamics Simulations.

Journal of chemical theory and computation·2026
Same author

Size and Cross-Linking Dependent Gold Nanoparticle Interactions with Dynamic Pectin Model Plant Cell Walls.

ACS nano·2026
Same author

Proximity-Induced Rewiring of Oncogenic Kinase Triggers Apoptosis.

ACS central science·2026
Same author

Advancing Reproducibility and Open Data in Theoretical and Computational Chemistry.

Journal of chemical theory and computation·2026
Same journal

From cyclic diaryl λ<sup>3</sup>-bromanes/chloranes to polyfuntionalized biarylsilanes <i>via</i> aryne σ-bonds.

Chemical science·2026
Same journal

Non-equilibrium formation of the elusive dibridged diboranyl (B<sub>2</sub>H<sub>5</sub>) radical and boranes in low-temperature diborane ices.

Chemical science·2026
Same journal

Visible-light-driven ruthenium-catalyzed hydrogenation of manganese nitride complexes to ammonia under ambient conditions.

Chemical science·2026
Same journal

Quantification of mesopore infiltration in a polymer-grafted metal-organic framework.

Chemical science·2026
Same journal

Enhanced and selective oxygen reduction by iron porphyrin with a biguanide residue in the second coordination sphere.

Chemical science·2026
Same journal

Excited-state orbital angular momentum enables all-optical molecular spin coherence.

Chemical science·2026
See all related articles

Related Experiment Video

Updated: Feb 10, 2026

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
09:11

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Published on: November 23, 2019

7.2K

Counting charges on membrane-bound peptides.

Alicia C McGeachy1, Emily R Caudill2, Dongyue Liang2

  • 1Department of Chemistry , Northwestern University , 2145 Sheridan Road , Evanston , IL 60660 , USA .

Chemical Science
|May 22, 2018
PubMed
Summary
This summary is machine-generated.

Quantifying peptide charge at interfaces is challenging. This study uses SHG, QCM-D, and NPS to measure lysine and arginine octamers on lipid bilayers, finding they neutralize surface charge and remain fully ionized.

More Related Videos

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
07:10

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

6.1K
Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

7.2K

Related Experiment Videos

Last Updated: Feb 10, 2026

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
09:11

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Published on: November 23, 2019

7.2K
In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
07:10

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

6.1K
Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

7.2K

Area of Science:

  • Biophysics
  • Surface Chemistry
  • Computational Chemistry

Background:

  • Quantifying peptide charge at solid/water interfaces is crucial but difficult.
  • Surface coverage and charge density are key parameters for understanding peptide-lipid interactions.

Purpose of the Study:

  • To determine the thermodynamics and electrostatics of l-lysine and l-arginine octamers interacting with supported lipid bilayers.
  • To provide reliable estimates of surface coverage and surface charge density for these peptides.

Main Methods:

  • Second Harmonic Generation (SHG) spectroscopy
  • Quartz Crystal Microbalance with Dissipation monitoring (QCM-D)
  • Nanoplasmonic Sensing (NPS) mass measurements
  • Atomistic simulations

Main Results:

  • Combined SHG/QCM-D/NPS yielded lower interfacial charge density estimates (0.12 ± 0.03 C m⁻² for Lys₈, 0.10 ± 0.02 C m⁻² for Arg₈) compared to poly-peptides.
  • Cationic peptides neutralized the negatively charged lipid bilayer surface.
  • Peptides remained fully ionized at the interface, similar to solution conditions.
  • Atomistic simulations revealed distinct conformations: Lys₈ favored a 'stand-up' orientation, while Arg₈ preferred a 'buried' conformation.
  • The Gouy-Chapman model was found to be semi-quantitatively valid for this system, with an apparent dielectric constant of ~30.

Conclusions:

  • The study successfully quantified peptide charge density at a solid/water interface.
  • Lysine and arginine octamers effectively neutralize lipid bilayer surface charge.
  • Conformational preferences influence peptide-interface interactions, impacting charge distribution.