Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.9K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.9K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

7.0K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.0K
Structural Protein Function01:56

Structural Protein Function

30.0K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
30.0K
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

807
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
807
Genomics02:02

Genomics

40.8K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.8K
Fruit Development, Structure, and Function01:58

Fruit Development, Structure, and Function

25.4K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural basis of RNA-guided DNA integration by type I CRISPR-associated transposases.

bioRxiv : the preprint server for biology·2026
Same author

Transposon end recognition and excision mechanisms of type I-F CRISPR-associated transposases.

bioRxiv : the preprint server for biology·2026
Same author

Programmable genome editing in human cells using RNA-guided bridge recombinases.

Science (New York, N.Y.)·2026
Same author

One-shot design of functional protein binders with BindCraft.

Nature·2025
Same author

Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons.

Nucleic acids research·2025
Same author

Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation.

Nature structural & molecular biology·2025
Same journal

Molecular Interplay of PARN and Telomerase: Tail Modifiers and Disease Implications.

Wiley interdisciplinary reviews. RNA·2026
Same journal

Exploring New Frontiers in Bone Metabolism: Role and Potential of lncRNA DANCR.

Wiley interdisciplinary reviews. RNA·2026
Same journal

Functional Inclusion of RNA Biology in the Tethered Extracellular Matrix.

Wiley interdisciplinary reviews. RNA·2026
Same journal

Structural and Functional Diversity of RNA-Containing Toxin-Antitoxin Systems.

Wiley interdisciplinary reviews. RNA·2026
Same journal

Promoter-Targeting RNA Technologies: An Epigenetic Strategy for Gene Activation and Gene Silencing.

Wiley interdisciplinary reviews. RNA·2026
Same journal

LncRNA PCAT18: Roles and Mechanisms in Human Cancers.

Wiley interdisciplinary reviews. RNA·2026
See all related articles

Related Experiment Video

Updated: Feb 10, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.8K

Cas9 versus Cas12a/Cpf1: Structure-function comparisons and implications for genome editing.

Daan C Swarts1, Martin Jinek1

  • 1Department of Biochemistry, University of Zurich, Zurich, Switzerland.

Wiley Interdisciplinary Reviews. RNA
|May 24, 2018
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas9 and CRISPR-Cas12a are powerful genome editing tools. This study compares their distinct structures and mechanisms, guiding the choice between Cas9 and Cas12a for specific gene editing applications.

Keywords:
CRISPR-CasCRISPR/CasCas12aCas9Cpf1RNA-guidedgenome editingnuclease

More Related Videos

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.8K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

5.1K

Related Experiment Videos

Last Updated: Feb 10, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.8K
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.8K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

5.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • CRISPR-Cas9 and CRISPR-Cas12a are programmable nucleases used for genome editing and gene regulation.
  • Cas9 is widely used, but Cas12a (formerly Cpf1) is an emerging alternative with distinct properties.

Purpose of the Study:

  • To compare the structural and mechanistic differences between Cas9 and Cas12a.
  • To discuss how these differences influence their activity and applications in genome editing.
  • To review recent uses of Cas12a as a genome editing tool.

Main Methods:

  • Comparative analysis of structural and mechanistic features of Cas9 and Cas12a.
  • Literature review of recent studies utilizing Cas12a for genome editing.

Main Results:

  • Cas9 and Cas12a possess different evolutionary origins, structures, and molecular mechanisms.
  • These distinctions impact their nuclease activity and suitability for specific genome editing tasks.
  • Cas12a offers a viable alternative to Cas9, with unique advantages for certain applications.

Conclusions:

  • Understanding the distinct features of Cas9 and Cas12a is crucial for selecting the optimal nuclease for genome editing.
  • Cas12a presents a promising tool for diverse gene editing applications.
  • Further research into Cas12a will expand its utility in molecular biology and biotechnology.