[PM2.5 and polluted gas exposure induced acute injury in rats'larynx membrane]

Objective:To study acute injury of rat's larynx membrane which were exposured to PM2.5 and polluted gas in serious air pollution. Method:Rats were divided into 3 groups of 5 animals each, the normal control group, gas exposured group, PM2.5 +gas exposured group. The control group served as the normal healthy control, rats of the gas exposured group had been exposured polluted gas in the A cabin, rats of PM2.5 +gas exposured group had been exposured PM2.5 and polluted gas in the B cabin. The total exposure time was six days. We collected specimen from 3 groups immediately after exposure. Histopathological changes in the rat's larynx membrane were assessed using hematoxylin and eosin staining(HE), the expression of IL-1β in laryngeal mucous membrane were assessed using immunohistochemical staining(IHC), and the ultrastructure changes were observed under TEM. Result:HE staining showed the rat's laryngeal mucosa cells in the normal control group had no obvious abnormalities, laryngeal mucosa of rats in the two experimental group had different degrees of cell proliferation, cell polarity change, etc. IHC staining showed that there's no obvious expression of IL-1β in laryngeal mucous membrane in the normal control group, and positive expression in the two experimental group. TEM showed the control group had no obvious abnormalities, the laryngeal mucosa cells in the two groups of experimental groups had been found irregular nucleus and damaged mitochondria. Conclusion:Exposed to PM2.5 and polluted gas in severe atmospheric pollution 6 days, the acute damage in rats laryngeal mucosa can be observed.