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Updated: Feb 10, 2026

Transoral Robotic Total Thyroidectomy and Bilateral Central Regional Lymph Node Dissection for Papillary Thyroid Carcinoma
Published on: September 15, 2023
[Investigate the expression of miRNA-203 in papillary thyroid carcinomaand its effect on cell proliferation]
Abstract:
Objective:To investigate the expression of miRNA-203 in papillary thyroid carcinoma(PTC)tissues and its correlation with clinical pathological parameters,explore its effect on cell proliferation of WRO cell. Method:Thirty cases of PTC tissues, paired normal tissues were collected in our hospital during 2013-2016. The expression of miRNA-203 was determined by qRT-PCR,then the relationship of miRNA-203 expression, clinical pathological parameters were analyzed.WRO cells were transfected with miRNA-203 mimics, then cell proliferation, cell cycle and concerned cyclin protein(CyD1,CyB1) were tested by MTT, flow cytometry and western blot. Result:Compared to the paired normal tissues,tumor tissues showed sifnificantly lower expression of miRNA-203. Upregulaion of miRNA-203 in WRO cells effectively reduced cell growth, G₂/M arrest. Mechanistically,in the miRNA-203-mimics-treated groups,cell-cycle-related proteins cyclin B1 was up-regulated, while cyclin D1 was down-regulated. Conclusion:miRNA-203 may play an anticarcinogenic effect in PTC. Upregulation of miRNA-203 is highly correlated with cell prolliferation, and maybe miRNA-203 is a potential targert for the treatment of thyroid carcinoma.
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