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Designs and methods for interfacing SFC with MS.

Abhijit Tarafder1

  • 1Waters Corporation, Milford MA 01757, USA.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|May 27, 2018
PubMed
Summary
This summary is machine-generated.

Supercritical Fluid Chromatography (SFC) coupled with Mass Spectrometry (MS) is now routine. This review compiles insights on SFC-MS interfacing, linking solutions to the challenge of mobile-phase compressibility.

Keywords:
CompressibleCouplingExpansionInterfacingMass spectrometryMobile-phaseSFC-MSSolventSupercritical fluid chromatography

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Supercritical Fluid Chromatography (SFC) coupled with Mass Spectrometry (MS) has become a standard technique in analytical laboratories.
  • Commercial solutions facilitate the wider adoption of SFC-MS technology.

Purpose of the Study:

  • To compile insights on describing and optimizing SFC-MS interfacing processes.
  • To connect these insights to the fundamental challenge of SFC mobile-phase compressibility.

Main Methods:

  • Review of historical research and development in SFC-MS interfacing.
  • Analysis of various interface designs and adopted measures (e.g., solvent mixing, heating).

Main Results:

  • SFC-MS interfacing presented unique challenges compared to LC-MS or GC-MS.
  • Specific solutions, including mobile-phase modification and heating, were developed to overcome these challenges.
  • Mobile-phase compressibility is identified as a key factor driving interface optimization.

Conclusions:

  • Decades of research have led to current SFC-MS capabilities.
  • Understanding and addressing mobile-phase compressibility is crucial for successful SFC-MS interfacing.