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Biomimetic Implant Surface Functionalization with Liquid L-PRF Products: In Vitro Study
Marco Lollobrigida1, Manuela Maritato2, Giuseppina Bozzuto3
1Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Rome, Italy.
Biomed Research International
|June 2, 2018
Summary
Liquid leucocyte- and platelet-rich fibrin (L-PRF) products effectively form dense fibrin clots on micro/nano-rough implant surfaces. This in vitro study shows enhanced fibrin coating on rougher surfaces, suggesting potential benefits for oral surgery applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Oral Surgery
Background:
- Platelet-rich fibrin (PRF) is an autologous blood concentrate used in oral surgery.
- Liquid formulations of PRF, such as leucocyte- and platelet-rich fibrin (L-PRF), are less understood.
- Investigating the interaction of liquid L-PRF with implant surfaces is crucial for optimizing its clinical use.
Purpose of the Study:
- To evaluate the in vitro behavior of different titanium implant surfaces when exposed to liquid L-PRF products.
- To compare the effects of two distinct liquid L-PRF formulations on implant surface interactions.
- To assess the influence of surface topography on fibrin clot formation.
Main Methods:
- Pure titanium discs with either micro/nano-rough or machined surfaces were used.
- Samples were treated with a platelet, lymphocyte, and fibrinogen liquid concentrate (PLyF) or a fibronectin/vitronectin-rich exudate, or a combination.
- Scanning electron microscopy (SEM) was employed to analyze the surface interactions and fibrin deposition.
Main Results:
- PLyF concentrate treatment resulted in dense fibrin networks and blood elements on implant surfaces.
- Fibronectin/vitronectin exudate treatment showed fewer blood cells compared to PLyF.
- Combined treatments and micro/nano-rough surfaces led to thicker fibrin layer formation and increased fibrin retention.
Conclusions:
- Liquid L-PRF products promote dense fibrin clot formation on micro/nano-rough implant surfaces in vitro.
- Fibronectin/vitronectin exudate may enhance fibrin-surface contact but is not essential for clot formation.
- Further research is needed to fully understand the properties and clinical benefits of liquid L-PRF.
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