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Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace
Huigang Hu1, Qiaoli Zhao1, Zhencai Pang1
1Ministry of Agriculture Key Laboratory of Tropical Fruit Tree Biology, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China.
International Journal of Biological Macromolecules
|June 4, 2018
Summary
Ultrasonic-assisted extraction optimized polysaccharide yield from mango pomace. Purified fractions (MG-1, MG-2, MG-3) demonstrated significant in vitro anticancer activity and apoptosis promotion.
Area of Science:
- Food Science and Technology
- Biochemistry
- Pharmacology
Background:
- Mango pomace is a rich source of bioactive compounds.
- Polysaccharides possess various health benefits, including anticancer properties.
- Efficient extraction methods are crucial for utilizing these compounds.
Purpose of the Study:
- To optimize ultrasonic-assisted extraction of polysaccharides from mango pomace.
- To purify and characterize novel polysaccharide fractions.
- To investigate the in vitro anticancer activities of these fractions.
Main Methods:
- Response surface methodology for extraction optimization.
- DEAE-52 cellulose and Sephadex G-100 chromatography for purification.
- HPGPC, HPLC, FT-IR, 1H NMR, and SEM for characterization.
- In vitro assays for anticancer activity and apoptosis induction.
Main Results:
- Optimized extraction conditions yielded 3.71% polysaccharides.
- Three novel polysaccharide fractions (MG-1, MG-2, MG-3) were isolated.
- Fractions showed significant dose-dependent anticancer activity against multiple cell lines.
- Apoptosis promotion was confirmed via nuclear morphological examination.
Conclusions:
- Optimized ultrasonic-assisted extraction is effective for mango pomace polysaccharides.
- Novel polysaccharide fractions exhibit promising anticancer potential.
- These findings support the use of mango pomace polysaccharides in biomedical and functional food applications.
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