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Quantitation of Endothelial Cell Adhesiveness In Vitro
Published on: June 18, 2015
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In-vitro transdentinal diffusion of monomers from adhesives
Eveline Putzeys1, Radu Corneliu Duca2, Lieve Coppens1
1KU Leuven BIOMAT, Department of Oral Health Sciences, University of Leuven & Dentistry University Hospitals Leuven, Kapucijnenvoer 7, 3000 Leuven, Belgium.
Journal of Dentistry
|June 5, 2018
Summary
Dental adhesives can release monomers that diffuse through dentin to the pulp. Lower molecular weight monomers and uncured adhesives show higher diffusion, impacting pulp exposure and biocompatibility.
Area of Science:
- Dental Materials Science
- Biomaterials Engineering
- Toxicology
Background:
- Adhesive biocompatibility is crucial due to proximity to the dental pulp.
- Understanding monomer diffusion through dentin informs potential adverse effects.
- High tubular density near the pulp necessitates careful assessment of adhesive interactions.
Purpose of the Study:
- To quantify transdentinal diffusion of residual monomers from dental adhesive systems.
- To assess monomer elution and penetration using an in-vitro pulp chamber model.
- To evaluate the influence of curing status on monomer diffusion.
Main Methods:
- Human third molar dentin disks (300 μm) were used in an in-vitro pulp chamber model.
- Two adhesive systems (OptiBond FL, Clearfil SE Bond) were tested.
- Monomer diffusion was quantified using ultra-performance liquid chromatography-tandem mass spectrometry.
Main Results:
- Several monomers (HEMA, CQ, BisGMA, GPDM, 10-MDP, UDMA) diffused through dentin.
- Lower molecular weight monomers exhibited greater diffusion than higher molecular weight ones.
- Uncured adhesives showed up to 10 times higher monomer diffusion compared to cured conditions.
Conclusions:
- This study quantified monomer diffusion across dentin, providing critical data on pulp exposure.
- Findings highlight the importance of complete adhesive curing to minimize monomer leakage.
- Results contribute to a better understanding of dental adhesive biocompatibility and pulp protection.
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