Metal-organic frameworks in proteomics/peptidomics-A review

Area of Science:

• Proteomics and Peptidomics
• Materials Science
• Analytical Chemistry

Background:

• Peptides play crucial roles in physiological processes.
• Analyzing global peptides in complex biological systems is challenging due to matrix interference.
• Metal-organic frameworks (MOFs) possess unique structural and surface properties beneficial for biomolecule analysis.

Purpose of the Study:

• To explore the application of MOFs in proteomics and peptidomics.
• To highlight MOFs' capabilities in peptide capture, detection, and mass spectrometry.
• To discuss the development of MOFs as stationary phases for peptide analysis.

Main Methods:

• Constructing MOFs with amino acids/peptides to impart biomolecular characteristics.
• Utilizing MOFs as solid adsorbents for capturing various peptide types (endogenous, glyco-, phosphopeptides).
• Developing MOFs as matrices for peptide ionization in mass spectrometry and as stationary phases for chromatographic analysis.

Main Results:

• MOFs demonstrate effectiveness in capturing and selectively detecting peptides.
• MOFs can be used as matrices to enhance peptide ionization in mass spectrometry.
• MOFs have been successfully developed into particulate and monolith stationary phases for peptide analysis.

Conclusions:

• MOFs show significant potential for advancing sensitive, selective, high-throughput, and in situ peptide analysis.
• Further research into MOFs presents opportunities for overcoming current challenges in proteomics and peptidomics.
• MOFs offer versatile applications ranging from sample preparation to chromatographic separation in peptide research.