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Coronary Circulation01:21

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The heart, an organ critical to survival, gets nourishment not from the blood it pumps but from a separate circulation system known as coronary circulation. This is the shortest circulation in the body and is responsible for supplying the heart with the nutrients it needs to function effectively.
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Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
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Label-free isolation of prostate circulating tumor cells using Vortex microfluidic technology.

Corinne Renier1, Edward Pao2, James Che1

  • 1Vortex Biosciences Inc., 1490 O'Brien Drive, Suite E, Menlo Park, CA 94025 USA.

NPJ Precision Oncology
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This study introduces a microfluidic Vortex Chip for efficient and pure isolation of circulating tumor cells (CTCs) from prostate cancer patients. The method enables downstream genetic analysis, identifying unique mutations for targeted therapy prediction.

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Area of Science:

  • Oncology
  • Biotechnology
  • Genomics

Background:

  • Liquid biopsies offer non-invasive cancer biomarker identification.
  • Circulating tumor cells (CTCs) provide genetic and phenotypic insights into tumor evolution.
  • Existing CTC isolation methods often lack purity and require extensive manual preparation.

Purpose of the Study:

  • To evaluate the microfluidic Vortex Chip for size-based isolation of CTCs.
  • To assess the purity, efficiency, and speed of CTC capture.
  • To determine the suitability of isolated CTCs for downstream genetic analysis and mutation detection.

Main Methods:

  • Utilized the microfluidic Vortex Chip for size-based CTC isolation from advanced prostate cancer patients.
  • Performed enumeration studies to quantify CTC capture efficiency and purity.
  • Analyzed isolated cells for epithelial and epithelial-mesenchymal transition markers.
  • Conducted targeted amplification and next-generation sequencing on isolated CTC DNA.

Main Results:

  • Achieved high purity (1.74–37.59%) and efficiency (1.88–93.75 CTCs/7.5 mL) in under 1 hour.
  • Identified CTCs in 80% of prostate cancer patients using an age-adjusted threshold.
  • Detected epithelial-mesenchymal transition markers in a subset of isolated cells.
  • Identified unique mutations in 10 of 15 patient samples via next-generation sequencing.

Conclusions:

  • The Vortex Chip provides a rapid, high-purity method for CTC isolation.
  • Isolated CTCs are suitable for downstream genetic analysis, including mutation detection.
  • This technology has potential for cancer prognosis, monitoring, and guiding targeted therapies.