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Overexpression of nicotinamide N-methyltransferase in HSC-2 OSCC cell line: effect on apoptosis and cell

Riccardo Seta1, Marco Mascitti1, Roberto Campagna1

  • 1Department of Clinical Sciences, Polytechnic University of Marche, Via Ranieri 65, 60131, Ancona, Italy.

Clinical Oral Investigations
|June 9, 2018
PubMed
Abstract

Insights

Nicotinamide N-methyltransferase (NNMT) promotes oral squamous cell carcinoma (OSCC) growth and survival. Targeting NNMT may offer a new therapeutic strategy for oral cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Oral squamous cell carcinoma (OSCC) remains a significant health challenge with stagnant survival rates.
  • Novel molecular targets are crucial for improving early diagnosis and treatment efficacy.
  • Nicotinamide N-methyltransferase (NNMT) has been identified as an enzyme upregulated in OSCC.

Purpose of the Study:

  • To investigate the role of NNMT in oral cancer cell metabolism and proliferation.
  • To explore the molecular mechanisms by which NNMT influences OSCC cell growth and apoptosis.
  • To evaluate NNMT and survivin ΔEx3 isoform expression in OSCC tissues.

Main Methods:

  • Transfection of HSC-2 cells with NNMT expression vector.
  • Cell proliferation assessment using MTT assay.
  • Real-time PCR to analyze gene expression (β-catenin, survivin, Ki-67).
  • Immunohistochemistry on OSCC tissue samples.

Main Results:

  • NNMT upregulation significantly enhanced in vitro cell growth.
  • A positive correlation was observed between NNMT and survivin ΔEx3 isoform expression in both cell lines and patient tissues.
  • NNMT appears to influence cell proliferation and anti-apoptotic pathways.

Conclusions:

  • NNMT is implicated in the proliferation and tumorigenic potential of OSCC.
  • The enzyme's involvement in cell growth and anti-apoptotic mechanisms suggests it as a potential therapeutic target.
  • Targeting NNMT could represent a novel strategy to improve survival rates for OSCC patients.
Keywords:
Cell growthNNMTOral cancerSurvivinTumorigenicity

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