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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
Published on: December 26, 2019
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Flow Cytometry to Evaluate Potential Developmental Toxicants in the Embryonic Stem Cell.
Valéria Maria Lara1, Kelly C Santos Roballo1, André Tadeu Gotardo2
1Veterinary Medicine Department, Faculty of Animal Science and Food Engineering, Universidade de São Paulo, Pirassununga, SP, Brazil.
Methods in Molecular Biology (Clifton, N.J.)
|June 14, 2018
Summary
Embryonic stem cells offer a powerful model for toxicology testing due to their differentiation potential. Flow cytometry enhances toxicological and tumorigenic analyses of these cells in suspension.
Area of Science:
- Stem Cell Biology
- Toxicology
- Cellular Analysis
Background:
- Embryonic stem cells (ESC) possess unlimited differentiation capacity, making them suitable for toxicology testing.
- Toxicological analysis using ESC is an increasingly important methodology in safety assessment.
- Evaluating cellular characteristics is crucial for understanding toxic effects.
Purpose of the Study:
- To highlight the utility of embryonic stem cells in toxicological assessments.
- To introduce flow cytometry as a key technique for ESC toxicology.
- To demonstrate the application of flow cytometry in analyzing cellular properties for toxicology and tumorigenicity.
Main Methods:
- Utilizing embryonic stem cells (ESC) as a model system.
- Employing flow cytometry (FC) for cell analysis.
- Analyzing cells in suspension to assess biological, physical, and chemical characteristics.
Main Results:
- Embryonic stem cells are a viable model for toxicology.
- Flow cytometry effectively analyzes cells in suspension.
- Flow cytometry facilitates the study of cellular characteristics relevant to toxicology.
Conclusions:
- Embryonic stem cells are a valuable tool in toxicological procedures.
- Flow cytometry is a highly effective method for cell toxicology and tumorigenic analyses.
- The combination of ESC and FC offers robust capabilities for safety and cancer research.
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