[Simultaneous determination of 6 kinds of food additives in drink by HPLC-MS/MS]

To establish a high performance liquid chromatographytandem mass spectrometry( HPLC-MS/MS) method for simultaneous determination of caffeine, benzoic acid, sorbic acid, acesulfame potassium, saccharin sodium and sodium cyclamate in drink.

The sample was extracted by ASPEC using extraction column( Pro Elut C_(18)500 mg/6 m L), dried by Turbo VapⅡ and dissolved in mobile phase. The supernatant was separated on Agilent SB-C_(18) column( 2. 1 mm × 50 mm, 1. 8 μm)using 0. 02 mmol/L ammonium acetate/methyle alcohol as mobile phase and then detected by HPLC-MS/MS using multiple reaction monitoring( MRM) in positive ionization and negative ionization mode.

The average recoveries were from 72. 6% to 100. 5%and the relative standard deviations( RSD) were from 0. 3% to 3. 1%( n = 6). The calibration curves showed a good linearity with correlation coefficients r > 0. 9990. The linear range for saccharin sodium was 8-800 μg/L and others were 2-200 μg/L. The detection limits for caffeine, benzoic acid, sorbic acid, acesulfame potassium and sodium cyclamate were 0. 001 mg/kg and 0. 004 mg/kg for saccharin sodium.

The method is specific, sensitive, easy, fast and suitable for the confirmation and quantification of caffeine, benzoic acid, sorbic acid, acesulfame potassium, saccharinsodium and sodium cyclamate in drink.