Related Experiment Video
Updated: Feb 9, 2026
27:58
Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
Published on: October 1, 2007
11.4K
User-defined local stimulation of live tissue through a movable microfluidic port
Megan A Catterton1, Austin F Dunn, Rebecca R Pompano
1Department of Chemistry, University of Virginia, Charlottesville, VA, USA. rpompano@virginia.edu.
Lab on a Chip
|June 16, 2018
Summary
This study introduces a novel microfluidic device for precise, localized fluid delivery to living tissue slices. The system enables targeted stimulation without direct tissue manipulation, enhancing in vitro research capabilities.
Area of Science:
- Biotechnology
- Microfluidics
- Tissue Engineering
Background:
- In vitro studies often use global stimuli, unlike localized in vivo responses.
- Existing microfluidic devices require manual tissue alignment, risking damage.
- Precise, localized stimulation is crucial for accurate tissue response studies.
Purpose of the Study:
- To develop a microfluidic device for user-defined, focal fluid delivery to tissue slices.
- To enable precise stimulation without manipulating the delicate tissue sample.
- To overcome limitations of fixed-port microfluidic systems.
Main Methods:
- A two-component SlipChip design with a mobile port beneath the tissue.
- A culture chamber with an array of ports separated by fluorocarbon oil from a delivery port.
- Physical modeling based on interfacial tension and flow resistance to predict delivery conditions.
Main Results:
- Reproducible aqueous solution delivery to tissue and gel samples.
- Convection as the primary factor controlling the width of the delivery region.
- No adverse effects on tissue slice viability during stimulation.
Conclusions:
- The novel microfluidic device allows precise, localized fluid delivery to tissue slices without mechanical manipulation.
- The system is validated by successful sequential stimulation of lymph node tissue.
- This platform holds potential for studying fluid flow effects in tissues and for local drug screening.
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