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Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

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Fluorescence activation with switchable oxazines.

Yang Zhang¹, Sicheng Tang, Ek Raj Thapaliya

¹Laboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146-0431, USA. fraymo@miami.edu.

Chemical Communications (Cambridge, England)

|June 16, 2018

Summary

This summary is machine-generated.

Researchers developed novel fluorescent probes that switch on with light or pH. These advanced probes enable precise bioimaging with subdiffraction resolution, cancer cell detection, and real-time tracking of cellular processes.

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Area of Science:

Chemical Biology
Photochemistry
Bioimaging

Background:

Spatiotemporal control of fluorescence is crucial for advanced imaging strategies.
Conventional fluorophores lack the ability to be switched on/off with external stimuli.
Developing stimuli-responsive fluorescent probes is essential for overcoming these limitations.

Purpose of the Study:

To design and synthesize novel photoresponsive and halochromic oxazine-based probes.
To enable fluorescence activation with external triggers like light or pH.
To achieve spatiotemporal control over fluorescence emission for enhanced bioimaging.

Main Methods:

Covalent linkage of photoresponsive or halochromic oxazines to fluorescent chromophores.
Utilizing photochemical and photophysical properties of oxazines for fluorescence modulation.
Employing light or pH as external stimuli to trigger spectral shifts and activate fluorescence.

Main Results:

Demonstrated fluorescence switching via light or pH stimuli, leading to a bathochromic shift.
Achieved bright fluorescence with infinite contrast and precise spatiotemporal control.
Enabled subdiffraction resolution imaging, selective cancer cell signaling, and real-time monitoring of translocating species.

Conclusions:

Structural designs for externally controlled fluorescence switching offer significant advantages over conventional fluorophores.
These novel probes are invaluable for implementing advanced bioimaging strategies.
The developed mechanisms pave the way for next-generation fluorescent probes in biological research.