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Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica

Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica

Published on: October 20, 2020

CRISPR/Cas9-based heritable targeted mutagenesis in Thermobia domestica: A genetic tool in an apterygote development

Takahiro Ohde¹, Yusuke Takehana², Takahiro Shiotsuki³

¹Division of Evolutionary Developmental Biology, National Institute for Basic Biology, 38 Nishigonaka Myodaiji, Okazaki, 444-8585, Japan; Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), 38 Nishigonaka Myodaiji, Okazaki, 444-8585, Japan; Department of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.

Arthropod Structure & Development

|June 17, 2018

Abstract:

Despite previous developmental studies on basally branching wingless insects and crustaceans, the evolutionary origin of insect wings remains controversial. Knowledge regarding genetic regulation of tissues hypothesized to have given rise to wings would help to elucidate how ancestral development changed to allow the evolution of true wings. However, genetic tools available for basally branching wingless species are limited. The firebrat Thermobia domestica is an apterygote species, phylogenetically related to winged insects. T. domestica presents a suitable morphology to investigate the origin of wings, as it forms the tergal paranotum, from which wings are hypothesized to have originated. Here we report the first successful CRISPR/Cas9-based germline genome editing in T. domestica. We provide a technological platform to understand the development of tissues hypothesized to have given rise to wings in an insect with a pre-wing evolution body plan.

Keywords:

ABC transporter Apterygote insect CRISPR/Cas9 Genome editing Thermobia domestica

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