SE33 locus as a reliable genetic marker for forensic DNA analysis systems

Area of Science:

• Forensic genetics
• Population genetics

Background:

• Genetic variation is crucial for individual identification in forensic investigations.
• Inconclusive results occur when DNA markers are missing, necessitating additional reliable markers.
• The SE33 locus exhibits high genetic variability and is used in some forensic systems.

Purpose of the Study:

• To evaluate the SE33 locus as a supplementary marker for forensic DNA profiling.
• To assess the utility of SE33 in enhancing the conclusiveness of DNA analysis.

Main Methods:

• Amplification of the SE33 locus using the PowerPlex ES Monoplex System.
• Genotyping of 204 individuals from the Pakistani population.
• Analysis of genetic and forensic parameters for the SE33 locus.

Main Results:

• Identification of 43 alleles at the SE33 locus, including three novel alleles.
• Significant forensic and genetic parameters, such as high power of discrimination and exclusion.
• Demonstrated high polymorphism information content.

Conclusions:

• The SE33 locus can enhance forensic DNA profiling, particularly for degraded samples, leading to more conclusive results.
• SE33 is a viable marker for Pakistani and related populations with shared ancestry across Western and South Asia.
• Its inclusion can improve the efficiency and reliability of forensic casework.