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10:51
Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
Published on: October 13, 2021
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Biomimetic enzyme cascade reaction system in microfluidic electrospray microcapsules.
Huan Wang1, Ze Zhao1, Yuxiao Liu1
1State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Science Advances
|June 21, 2018
Summary
This study presents a novel multienzyme system using hydrogel microcapsules to mimic cellular compartments. This biomimetic system offers a promising approach for enzyme replacement therapy and biocatalysis.
Area of Science:
- Biomimetic chemistry
- Materials science
- Enzyme engineering
Background:
- Mimicking subcellular compartments with enzymes can restore lost cellular functions.
- Cellular architectures inspire novel multienzyme systems for biocatalysis.
Purpose of the Study:
- To develop a novel multienzyme system using hollow hydrogel microcapsules with enzymatic inverse opal particles.
- To create a system with controllable enzyme encapsulation and enhanced stability.
Main Methods:
- Utilized microfluidic electrospray for precise enzyme encapsulation.
- Incorporated flexible enzymatic inverse opal particles for structural color and enzyme arrangement.
- Employed hollow hydrogel microcapsules to protect enzymes from proteolysis.
Main Results:
- Developed a multienzyme system with controllable enzyme number, type, and spatial arrangement.
- Demonstrated enhanced enzyme stability against proteolysis within hydrogel shells.
- Showcased the system's potential as a cascade biocatalyst, reducing alcohol levels.
Conclusions:
- The developed multienzyme system serves as an ideal platform for complex biocatalysis.
- This approach offers a promising strategy for biomimicking organelle functions.
- The system provides a potential alternative antidote and prophylactic for alcohol intoxication.
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