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Updated: Feb 8, 2026

Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels
Published on: August 10, 2010
Unprecedented Acid-Promoted Polymerization and Gelation of Acrylamide: A Serendipitous Discovery
Siew Yin Chan1,2, Shermin S Goh2, Qingqing Dou2
1School of Science, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia.
Abstract:
Dilute acid polymerizes degassed, aqueous acrylamide with concomitant gelation, without the need for added free radical initiator or cross-linking agent. This reaction is accelerated by sonication or UV irradiation, but inhibited by adventitious oxygen or the addition of a free radical inhibitor, suggesting an acid-accelerated free radical process. The resulting hydrogels are thixotropic in nature and partially disrupted by the addition of chaotropic agents, indicating the importance of hydrogen bonding to the 3D network. This discovery was made while trying to prepare pectin-polyacrylamide hydrogels. We observed that pectin initiated the gelation of acrylamide, but only if the aqueous pectin samples had a pH lower than ca. 5.
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