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Updated: Feb 8, 2026

Generation of Human 3D Lung Tissue Cultures 3D-LTCs for Disease Modeling
Published on: February 12, 2019
Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease
Dan C Wilkinson1, Michael Mellody2, Luisa K Meneses3
1Department of Materials Science and Engineering, UCLA, Los Angeles, California.
Abstract:
This unit describes a protocol for generation of lung organoids. A lung organoid is a 3D cell/hydrogel composite that resembles the morphology and cellular composition of the human distal lung. These tissue-engineered constructs provide an in vitro model of human lung and are best suited for disease modeling applications. The organoid generation methodology is flexible, allowing for easy scalability in the number of organoids produced and in the ability to accommodate a wide range of cell types. © 2018 by John Wiley & Sons, Inc.
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