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Viral delivery of genome-modifying proteins for cellular reprogramming
1Department of Biomedicine, Aarhus University, DK-8000 Aarhus C, Denmark.
Current Opinion in Genetics & Development
|June 22, 2018
Summary
Virus-based gene therapy vehicles can be adapted for genome editing. Emerging virus-based protein delivery offers a short-term solution for precise genetic modification, unlike traditional gene therapies.
Area of Science:
- Biotechnology
- Molecular Biology
- Gene Therapy
Background:
- Virus-based gene vehicles are established for genetic therapies.
- Genome engineering tools require transient activity, unlike persistent gene expression in conventional therapies.
Purpose of the Study:
- To explore the potential of virus-based systems for transient genome engineering.
- To evaluate virus-based protein delivery as an alternative to viral vectors for genome editing.
Main Methods:
- Adaptation of viral vector systems for genome editing.
- Implementation of regulatable gene expression or self-limiting circuits.
- Development of virus-based protein delivery technologies.
Main Results:
- Viral vectors show potential for in vitro and in vivo genome editing.
- Need for controlled expression systems to limit exposure to genome-modifying enzymes.
- Virus-based protein delivery provides transient enzymatic activity in transduced cells.
Conclusions:
- Virus-based protein delivery may offer an effective method for transient genome engineering.
- This approach could overcome limitations of persistent gene expression from viral vectors.
- Further research is needed to optimize virus-based protein delivery for genome editing applications.
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