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Updated: Feb 8, 2026

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
Published on: October 22, 2012
[Effect of NADPH oxidase inhibitor apocynin on the exercise induced proteinuria in rats]
Li-Na Chen1, Gang Zhou1, le Wu1
1College of Sport, Hunan University, Changsha 410082, China.
Objective:
To investigate the effect of NADPH oxidase inhibitor apocynin on exercise-induced proteinuria and its related mechanism.
Methods:
Thirty-two SD rats were randomly divided into the control group (group C), control + drug group (group CA), exhaustive exercise group (group E), exhaustive exercise + drug group (group EA). The rats were administrated apocynin at 10 mg/kg weight,once a day for three days, and one hour after the drug injection, a one-time exhaustive exercise was performed. After exhaustive exercise, urine protein, blood urea nitrogen (BUN), and kidney reactive oxygen species (ROS) concentration, NOS activity, NOS and 3-NT concentration were detected.
Results:
In comparison to control group urinary protein (UP), ROS, inductible nitric oxide synthase (iNOS), 3-NT levels increased significantly in group E while those in group EA did not change.
Conclusions:
The elevated renal NADPH oxidase activity by exhaustive exercise induced ROS that can rapidly react with NO, and then produces excess peroxynitrite, which contributes to occurrence of exercise-induced proteinuria.
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