Related Experiment Video
Updated: Feb 8, 2026

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells
Published on: February 12, 2021
[The effect of rosuvastatin therapy on CCR2 expression in mononuclear cells and its upstream pathway]
Rui-Xue Du1, Ping Ye1, Guang-Tao Yan2
1Department of Geriatric Cardiology, Chinese PLA General Hospital, Beijing 100853.
Objective:
To investigate the effect of rosuvastatin therapy on C-C chemokine receptor(CCR2)expression in mononuclear cells in patients with carotid atherosclerosis and explore the possible upstream mechanism.
Methods:
Twenty patients without previous statin treatment were enrolled. Rosuvastatin were given 5 to 20 mg/day for 3 months. At baseline and 12 weeks, lipid profile and plasma monocyte chemotactic protein-1 (MCP-1) levels were examined. The mRNA and protein expressions of CCR2 in the mononuclear cells were measured with RT-PCR and flow cytometry, respectively. The mRNA and protein expression of peroxidase proliferator-activated receptor(PPAR β) were detected with RT-PCR and Western blot, respectively.
Results:
After 3-months rosuvastatin treatment, the patients' low-density lipoprotein cholesterol (LDL-C) levels decreased significantly (P<0.01). Compared with baseline, the mRNA and protein expressions of CCR2 in the mononuclear cells showed significantly decrease, as well as plasma MCP-1 levels (P<0.05). Both mRNA and protein expressions of PPAR β in the mononuclear cells increased (P<0.05).
Conclusions:
Rosuvastatin may attenuate MCP-1/CCR2 through PPARβ upstream pathway.
More Related Videos
10:34Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Published on: April 14, 2010
09:45Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Published on: January 1, 2017
Related Concept Videos
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
Cell Specific Gene Expression
Cell Specific Gene Expression
Gene Therapy
Stem Cell Therapy for Tissue Regeneration
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability