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Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
23.7K
Good-quality blastocysts derived from vacuolized morulas show reduced viability
Richard B Mayer1, Omar Shebl1, Peter Oppelt2
1Department of Gynecology, Obstetrics, and Gynecologic Endocrinology, Kepler University, Linz, Austria.
Fertility and Sterility
|June 25, 2018
Summary
Late vacuolization in day 4 embryos negatively impacts blastocyst formation and live birth rates. This finding suggests vacuolization is a significant predictor of reduced fertility outcomes in assisted reproductive technologies.
Area of Science:
- Embryology
- Reproductive Medicine
- In Vitro Fertilization (IVF)
Background:
- Spontaneous vacuolization in human embryos on day 4 of development is not well understood.
- Its role as an artifact or a developmental pathway requires clarification.
- Understanding vacuolization is crucial for improving IVF success rates.
Purpose of the Study:
- To determine if late spontaneous vacuolization on day 4 is an artifact or an alternative method of blastocele formation.
- To analyze the impact of this vacuolization on pregnancy and live birth outcomes.
Main Methods:
- A prospective observational study involving 424 patients undergoing IVF.
- Patients were grouped based on the presence and extent of spontaneous vacuolization on day 4 morulas.
- Interventions included screening for day 4 vacuoles and single-blastocyst transfer.
Main Results:
- Group 1 (all morulas affected) showed reduced blastocyst formation rates on day 5 (P<.01).
- This group also had significantly fewer good-quality blastocysts (P<.05).
- Pregnancy (P<.001) and live birth (P<.01) rates were significantly lower in Group 1 compared to other groups.
Conclusions:
- Late-onset vacuolization around the compaction stage is a negative predictor of blastocyst formation.
- This phenomenon significantly impacts reproductive outcomes, including pregnancy and live birth rates.
- Vacuolization on day 4 should be considered when assessing embryo viability in IVF.
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