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The ITS2 Database
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PtRFdb: a database for plant transfer RNA-derived fragments.

Nikita Gupta1, Ajeet Singh1, Shafaque Zahra1

  • 1Lab #202, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi, India.

Database : the Journal of Biological Databases and Curation
|June 26, 2018
PubMed
Summary

Researchers have developed PtRFdb, a new database cataloging plant transfer RNA-derived fragments (tRFs). This resource aids in the study and characterization of these small RNAs across various plant species.

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Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Plant Science

Background:

  • Transfer RNA-derived fragments (tRFs) are small RNAs generated from transfer RNAs (tRNAs).
  • While extensively studied in animals, tRFs in plants remain underexplored.
  • A centralized resource is needed for plant tRF research.

Purpose of the Study:

  • To introduce PtRFdb, a comprehensive database for plant tRFs.
  • To facilitate the exploration and characterization of plant tRFs.
  • To provide a valuable resource for the scientific community studying plant small RNAs.

Main Methods:

  • Analysis of 1344 small RNA sequencing datasets from 10 diverse plant species.
  • Identification and cataloging of unique tRFs, including tRF-1, tRF-3, and tRF-5 types.
  • Development of a user-friendly database with search, browse, and download functionalities.

Main Results:

  • Identification of 5607 unique plant tRFs across 10 species.
  • PtRFdb contains detailed information including sequence, genomic location, and associated metadata.
  • The database offers advanced search options and data download capabilities.

Conclusions:

  • PtRFdb is a unique and valuable resource for plant tRF research.
  • The database will aid in the validation and further characterization of plant tRFs.
  • Facilitates comparative studies and functional analysis of plant tRFs.