Related Experiment Video
Updated: Feb 8, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
[Molecular Identification and Variation Analysis of the NSP2 and ORF5 Genes of PRRSV in Henan Province from 2014 to
Abstract:
This study aimed at investigating the molecular epidemiology and genetic variation of PRRSV based on the detection of 250 clinical samples collected from 118 farms from 2014 to 2015in different regions of Henan Province by RT-PCR. The NSP2 and ORF5genes of the PRRSV-positive samples were sequenced and analyzed. The results showed that 58 samples were positive for PRRSV, with a positive rate of 23.2%.A total of 29NSP2 and 31ORF5genes were obtained. The phylogenetic analysis revealed that most of the prevalent strains belonged to the North American genotype. Among the 58 positive samples,14 strains were highly homologous with HP-PRRSV, another 15 samples were highly homologous with the North American prevalent strain, and NADC30 contained a discontinuous deletion of 131 amino acids in Nsp2,which had been recently reported in China and Korea. This study showed that the HP-PRRSV and NADC30-Like strains are presently the dominant strains in Henan,particularly in comparison with the results from 2012 to 2013.Moreover,the NADC30-Like strains accounted for a higher percentage. In addition, both the NSP2 and ORF5genes had significant variations, suggesting that more attention should be continuously paid to monitor the pathogenic epidemiology and genetic variation of PRRSV. Furthermore, additional research should be conducted regarding the mechanism of pathogenicity and immunological suppression of PRRSV to provide a reference for the research and development of vaccines and antiviral drugs.
More Related Videos
05:56Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Published on: November 12, 2020
07:00Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas
Published on: February 28, 2019
Related Concept Videos
What is Variation?
The range, standard deviation, standard error, and variance are the different measures of variation.
Range: The range is the difference between its maximum and...
Variation
When independent and dependent variables are plotted on a scatter plot, the slope of a line is a value that describes the rate of change between the two...
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Variation of Atmospheric Pressure
Assuming the air temperature is constant at a given altitude and that the ideal gas law of thermodynamics describes the atmosphere to a good approximation, one can find the variation of atmospheric pressure with height.
Let p(y) be the atmospheric pressure at...
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Variation: Normal Distribution, Range, and Standard Deviation