Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Growth Measurement: Direct Methods

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...

mRNA Stability and Gene Expression

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability

Directing Effect of Substituents: meta-Directing Groups

Directing Effect of Substituents: meta-Directing Groups

Substituents on the benzene ring that direct an incoming electrophile to undergo substitution at the meta position are called meta directors. All meta directors either have a positive charge on the atom directly bonded to the ring or a partial positive charge. These groups function by withdrawing electrons from the ring through inductive and resonance effects. Consider the carbocation intermediates formed upon the addition of an electrophile on nitrobenzene at the...

Proteins: From Genes to Degradation

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...

Gene Evolution - Fast or Slow?

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...

Gene Flow

Gene Flow

Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optimizing single-lead ECG axis for AI-based detection of myocardial diseases.

NPJ cardiovascular health·2026

Same author

Convolutional Neural Network Models Leverage Morphological Rather Than Temporal Features to Detect Myocardial Diseases From 12-Lead Electrocardiograms.

Circulation. Arrhythmia and electrophysiology·2025

Same author

Software-driven chronic disease management: Algorithm design and implementation in a community-based blood pressure control pilot.

SAGE open medicine·2024

Same author

Artificial Intelligence and Health Equity: Opportunities and Obstacles.

JACC. Advances·2024

Same author

Understanding activity and physiology at scale: The Apple Heart & Movement Study.

NPJ digital medicine·2024

Same author

Artificial Intelligence and Machine Learning in Cardiology.

Circulation·2024

Search research articles

Related Experiment Video

Updated: Feb 8, 2026

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

A Rapid Method for Directed Gene Knockout for Screening in G0 Zebrafish.

Roland S Wu¹, Ian I Lam², Hilary Clay²

¹Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA; Division of Cardiology, Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.

Developmental Cell

|July 6, 2018

Summary

This study introduces a rapid CRISPR/Cas9 gene knockout system for zebrafish, enabling efficient generation of null phenotypes in G0 embryos. This method accelerates genetic screening for developmental and disease studies in zebrafish models.

Keywords:

CRISPR Cas9 G0 gene editing mutagenesis reverse genetic screen zebrafish

More Related Videos

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Published on: October 16, 2015

Gene Trapping Using Gal4 in Zebrafish

Gene Trapping Using Gal4 in Zebrafish

Published on: September 29, 2013

Related Experiment Videos

Last Updated: Feb 8, 2026

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Published on: October 16, 2015

Gene Trapping Using Gal4 in Zebrafish

Gene Trapping Using Gal4 in Zebrafish

Published on: September 29, 2013

Area of Science:

Genetics
Developmental Biology
Zebrafish Models

Background:

Zebrafish are valuable for genetic studies, but creating stable mutant lines is time-consuming.
Existing reverse genetic methods in zebrafish require significant time for stable mutant line generation.

Purpose of the Study:

To develop a rapid gene knockout system in zebrafish that consistently produces null phenotypes in G0 embryos.
To create a comprehensive resource for efficient gene targeting in zebrafish.

Main Methods:

Utilized CRISPR/Cas9 ribonucleoprotein complexes for yolk injection in zebrafish embryos.
Employed sets of four guides to redundantly target single genes, achieving high efficiency.
Developed a lookup table for four-guide sets targeting over 21,000 zebrafish genes.

Main Results:

Achieved germline-transmitted knockout phenotypes in >90% of G0 zebrafish embryos for 8 tested genes.
Observed both early embryonic and stable adult phenotypes.
Successfully targeted 50 cardiomyocyte transcriptional regulators, identifying a role for zbtb16a in cardiac development.

Conclusions:

The described CRISPR/Cas9 system enables rapid generation of knockout phenotypes in G0 zebrafish, significantly reducing time for genetic screening.
This platform facilitates efficient screening of genes in zebrafish for roles in development, physiology, and disease.
A validated lookup table of guide sets enhances the accessibility and utility of this rapid gene knockout system.