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Published on: July 31, 2012
Dual-functional probe based on rhodamine for sequential Cu2+ and ATP detection in vivo
Xilang Jin1, Jingkai Gao2, Pu Xie1
1School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an, 710032, Shaanxi, China.
Abstract:
A rhodamine-based fluorescent probe for Cu2+ and ATP has been designed. The fluorescence intensity/absorbance was significantly enhanced upon the addition of Cu2+ owning to the opening of the spiro-ring of rhodamine, which quickly returned to the original level due to the reconstruction of the probe by the reacting with ATP. Cu2+/ATP-induced fluorescent intensity/aborbance changes showed a good linear relationship with the concentration of Cu2+/ATP in the range of 2-20 μM/0-10 μM with a detection limit of 0.1 μM/1.0 μM. The proposed method is simple in design and fast in operation, and is suitable for the reversible monitoring of Cu2+ and ATP in bioanalytical applications.
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