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Development and Interlaboratories Validation of Event-Specific Quantitative Real-Time PCR Method for Genetically
Litao Yang1, Yu Yang1, Wujun Jin2
1National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology , Shanghai Jiao Tong University , Shanghai 200240 , China.
Abstract:
The transgenic rice G6H1 was a new event with the traits of herbicide-tolerance and insect-resistant. Herein, we developed one event-specific real-time PCR method with high specificity and sensitivity for G6H1 event quantitative analysis, and validated its performance on practical samples quantification through a collaborative ring trial. A total of eight laboratories participated in this validation and quantified three blind G6H1 powder samples including DNA extraction and real-time PCR analysis. The statistically analyzed results from returned data confirmed its high PCR efficiency and good linearity, trueness, and precision, indicating that the developed G6H1 real-time PCR assay was accurate, reliable, and comparable for G6H1 identification and quantification.
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