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A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo
Published on: May 16, 2020
Rat Model of Cardiotoxic Drug-Induced Cardiomyopathy
Takehiro Nakahara1,2,3, Takashi Tanimoto4,5, Artiom D Petrov4
1Division of Cardiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. takehironakahara@gmail.com.
Abstract:
Cardiotoxicity from cancer drugs remains a clinical problem. To find reliable markers of cardiotoxicity, animal models were proposed and potential new diagnostic markers have been actively investigated using these models. Here we describe our protocols, using male Sprague-Dawley rats, for inducing cardiomyopathy by single injection of high-dose doxorubicin (5-10 mg/kg) or multiple injections (2-4 times) of low-dose doxorubicin (2.5 mg/kg) with combined single injection of trastuzumab (10 mg/kg). The cardiotoxicity is evaluated by imaging modalities (echocardiography and nuclear imaging), serum troponin levels, and histopathological analyses.
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