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Naringenin inhibits prostate cancer metastasis by blocking voltage-gated sodium channels

Hatice Gumushan Aktas1, Tuba Akgun1

  • 1Biology Department, Faculty of Arts & Sciences, Harran University, Osmanbey Campus, Sanliurfa, Turkey.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|July 11, 2018
PubMed

Insights

Naringenin, a natural compound, was found to reduce the movement of MAT-LyLu cancer cells. It achieves this by decreasing the expression of the SCN9A gene, which encodes a key sodium channel involved in cell metastasis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • MAT-LyLu cells exhibit metastatic behavior linked to overexpression of voltage-gated sodium channels.
  • Understanding the role of these channels in cell motility is crucial for developing anti-metastatic strategies.

Purpose of the Study:

  • To investigate the effects of naringenin on MAT-LyLu cell motility.
  • To determine if naringenin affects the expression of the SCN9A gene, which encodes the Nav1.7 sodium channel.

Main Methods:

  • Cell proliferation and toxicity assays were performed to establish non-toxic naringenin concentrations.
  • Wound healing and transwell invasion assays assessed naringenin's impact on lateral and vertical cell motility.
  • Real-time quantitative polymerase chain reaction (RT-qPCR) measured SCN9A gene expression.

Main Results:

  • Naringenin at high concentrations (75 μM) inhibited cell proliferation.
  • Low concentrations of naringenin (5 and 10 μM) significantly decreased MAT-LyLu cell movement.
  • 10 μM naringenin reduced SCN9A gene expression at the mRNA level, correlating with decreased cell motility.

Conclusions:

  • Naringenin exhibits anti-motility effects on MAT-LyLu cells.
  • These effects are associated with the suppression of SCN9A gene expression.
  • Naringenin may act by directly or indirectly blocking voltage-gated sodium channels encoded by SCN9A.
Keywords:
MAT-LyLu cellsMetastasisNaringeninProstate cancerSCN9AVoltage-gated sodium channels

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