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Detection of parvovirus B19 in selected high-risk patient groups & their phylogenetic & selection analysis

Kumaran Vadivel1, Ramamurthy Mageshbabu1, Sathish Sankar1

  • 1Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital & Research Centre, Vellore, India.

The Indian Journal of Medical Research
|July 13, 2018
PubMed

Insights

Human parvovirus B19V (B19V) infection was detected in 1.13% of patients with febrile illness, arthropathies, or end-stage renal disease. Phylogenetic analysis confirmed all detected B19V strains belonged to genotype 1 with low genetic diversity.

Area of Science:

  • Virology
  • Genetics
  • Epidemiology

Background:

  • Human parvovirus B19V (B19V) is linked to various conditions including erythema infectiosum, aplastic crisis, hydrops fetalis, and arthritis.
  • The prevalence and genetic diversity of B19V in specific patient populations require further investigation.

Purpose of the Study:

  • To determine the presence of B19V infection in childhood febrile illnesses.
  • To investigate the association of B19V with arthropathies.
  • To assess B19V infection in adult patients with end-stage renal disease (ESRD) on dialysis.
  • To analyze the genetic diversity of B19V strains.

Main Methods:

  • A nested polymerase chain reaction (nPCR) assay targeting the VP1/VP2 region was employed.
  • B19V DNA detection was performed on 618 patients and 100 healthy controls.
  • Phylogenetic analysis of nucleotide and amino acid sequences was conducted to compare with global strains.

Main Results:

  • Seven out of 618 (1.13%) samples tested positive for B19V.
  • The positive cases included patients with non-traumatic arthropathies (4), ESRD (2), and febrile illness (1).
  • Phylogenetic analysis revealed all seven sequences belonged to genotype 1, exhibiting low genetic diversity and similar clustering patterns.

Conclusions:

  • B19V infection is present in patients with febrile illnesses, arthropathies, and ESRD.
  • The detected B19V strains are predominantly genotype 1, with limited genetic variation.
  • Indian B19V strains showed closer genetic relationships to Tunisian strains than other global strains.
Abstract

Keywords:
Arthropathy - end-stage renal disease - febrile illness - nested polymerase chain reaction - parvovirus B19 - polymerase chain reaction

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