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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
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Highly efficient RNA-guided base editing in rabbit.

Zhiquan Liu1, Mao Chen1, Siyu Chen1

  • 1Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, Jilin University, Changchun, 130062, China.

Nature Communications
|July 15, 2018
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Summary
This summary is machine-generated.

Cytidine and adenine base editors (CBEs and ABEs) efficiently create targeted point mutations in rabbit embryos. This technology enables precise generation of rabbit models for human diseases.

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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
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Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Editing

Background:

  • Cytidine base editors (CBEs) and adenine base editors (ABEs) are powerful tools for precise DNA base conversions.
  • These systems utilize deaminases fused to Cas9 nickase to achieve specific nucleotide changes.

Purpose of the Study:

  • To evaluate the efficiency and applicability of CBEs and ABEs in rabbit embryos for generating disease models.
  • To assess the performance of different base editor versions, including BE3, ABE7.10, and BE4-Gam.

Main Methods:

  • Application of BE3 and ABE7.10 base editing systems in rabbit blastocysts and Founder (F0) rabbits.
  • Induction of specific mutations, including nonsense, missense, and RNA mis-splicing, to mimic human pathologies.
  • Utilizing BE4-Gam, an updated BE3 version, to assess indel frequencies and product purity.

Main Results:

  • Achieved high targeted mutation efficiencies: 53-88% for BE3 and 44-100% for ABE7.10 in rabbit embryos.
  • Successfully induced various mutations and RNA mis-splicing, accurately modeling human diseases in rabbits.
  • BE4-Gam demonstrated reduced indel frequencies and higher product purity in rabbit blastocysts.

Conclusions:

  • CBEs and ABEs provide a simple and efficient method for introducing targeted point mutations in rabbits.
  • This gene editing strategy is effective for generating precise rabbit disease models.
  • The findings support the use of base editing for advancing genetic research and disease modeling in rabbits.