[Cultivation of bovine pestivirus in organ culture]

Attempts were made to culture strains of the bovine pestivirus in organ cultures of tracheal rings. Contrary to other scientists the authors used roller organ cultures of calf tracheal rings. The development of the virus in the cultures was judged by its presence in the maintaining medium through ELISA or by titration in cell cultures of fetal calf kidney. It was found that the two strains tested could be replicated in organ cultures of calf trachea, the intensity of multiplication being strongest between the third and the sixth day as demonstrated by ELISA. It was also established that the various batches of tracheal tissue had varying susceptibility.