FORMATION OF BIOFILMS BY BACTERIA EXCRETED FROM CHRONIC ANAL FISSURE AND THE INFLUENCE OF THE DIRECT CURRENT ELECTRIC...

Chronic anal fissures (CAF) are considered to be the illness with chronic course and characterized by frequent relapses. Although today the attention of researchers concerning the role of biofilm bacterial forms in pathogenesis of this pathology remains inadequate. Тherefore we investigated biofilms density of bacteria isolated from CAF and determined the current density possessing an optimal bactericidal effect on bacteria and destruct microbial biofilms. Bacteria isolated from CAF in monoculture manifest stronger adhesive properties and exopolysaccharide biofilm matrix in them is denser, which might provide better protection for microbe cells against environmental factors and antimicrobial drugs. To determine the optimal current strength essential for galvanization of CAF the effect of direct current electric field of different density was examined. The action of direct current electric field with the density of 0,025 mА/cm2 did not produce bactericidal effect on biofilm cells, although ruined biofilm matrix, which density decreased in 1,5 times as much in an average. In case the current density increased to 0,05-0,1 mА/cm2 biofilm matrix was ruined more intensively, its density decreased from high to average and low. It killed bacteria, their number decreased in the ruined biofilm from 10,7 to 56,4 times. The antibiotic fluoroquinolones and antiseptic Dioxisolum were found to produce the best effect on biofilm forms of bacteria. Although none of the examined antibacterial drugs possessed a complete bactericidal action, since bacteria were protected by biofilm matrix of high density causing resistance to pharmacological agents. Considering the mentioned above we recommend to supplement a comprehensive antibacterial therapy of CAF with intra-tissue electrophoresis with the current density 0,05-0,1 mА/cm² with antiseptic, and antibacterial therapy should be administered after preliminary detection of sensitivity of isolated microorganisms in biofilm from CAF to antibiotics and antiseptics.