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MORPHOLOGICAL ASSESSMENT OF NO-SYNTHASE DISTRIBUTION IN OVERACTIVE BLADDER AND STRESS URINE INCONTINENCE IN ANIMAL
O Iatsyna1, S Vernygorodskyi2, F Kostyev2
1Odessa National Medical University, Ministry of Public Health; Vinnytsia National Pirogov Memorial Medical University, Ukraine.
Georgian Medical News
|July 24, 2018
Summary
This study investigated nitric oxide synthase (NOS) in bladder conditions. Mirabegron and Quercetin therapies, with hormones, normalized NOS expression and reduced inflammation in overactive bladder and stress urinary incontinence.
Area of Science:
- Urology
- Pharmacology
- Cell Biology
Background:
- Overactive bladder (OAB) and stress urinary incontinence (SUI) involve complex physiological changes.
- Nitric oxide synthase (NOS) plays a crucial role in bladder function and dysfunction.
Purpose of the Study:
- To evaluate immunohistochemically the distribution of nitric oxide synthase (NOS) fractions in the bladder wall.
- To assess the effects of Mirabegron, Spasmex, Quercetin, Testosterone, and Estradiol on NOS expression in OAB and SUI models.
Main Methods:
- Immunohistochemical analysis of inducible (iNOS), endothelial (eNOS), and neuronal (nNOS) synthase fractions.
- Experimental models of overactive bladder (OAB) and stress urinary incontinence (SUI) were utilized.
Main Results:
- OAB and SUI exhibited increased iNOS expression in interstitial cells and decreased eNOS/nNOS expression.
- Mirabegron and Quercetin, combined with Testosterone and Estradiol, stabilized eNOS/nNOS and reduced iNOS expression.
- Spasmex showed less efficacy in modulating NOS fractions compared to the other combined therapies.
Conclusions:
- Therapies combining Mirabegron or Quercetin with Testosterone and Estradiol show promise in restoring normal NOS balance in OAB and SUI.
- These findings suggest a potential therapeutic strategy for managing bladder dysfunction by targeting NOS pathways.
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