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Updated: Feb 7, 2026

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
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Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Published on: November 20, 2017

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Luciferase Complementation Assay for Protein-Protein Interactions in Plants

Zhaoyang Zhou1, Guozhi Bi1, Jian-Min Zhou1

  • 1State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Current Protocols in Plant Biology
|July 25, 2018
PubMed
Summary

This study presents a luciferase complementation assay protocol for real-time plant protein-protein interaction analysis. The method offers quantitative, high-throughput, and scalable detection of these crucial cellular interactions.

Keywords:
Nicotiana benthamianainteractomeluciferase complementation assayprotein-protein interactionssplit luciferase assay

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