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Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates
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Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Published on: July 6, 2013

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Cytomegalovirus Infection: Mouse Model

Ilija Brizić1, Berislav Lisnić1, Wolfram Brune2

  • 1Department of Histology and Embryology and Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.

Current Protocols in Immunology
|July 26, 2018
PubMed

Insights

This study details methods for infecting mice with murine cytomegalovirus (MCMV). It covers virus propagation, preparing virulent strains, and quantifying viral loads using plaque assays for research applications.

Area of Science:

  • Virology
  • Immunology
  • Animal Models

Background:

  • Murine cytomegalovirus (MCMV) is a significant pathogen in research settings.
  • Standardized protocols are essential for reproducible MCMV infection studies.

Purpose of the Study:

  • To provide comprehensive procedures for MCMV infection in mice.
  • To establish methods for virus propagation and titration.

Main Methods:

  • Infection of newborn and adult mice with MCMV.
  • Propagation of MCMV in cell cultures and isolation from salivary glands.
  • Plaque assay for MCMV titration in tissues and stocks.
  • Preparation of murine embryonic fibroblasts for MCMV work.

Main Results:

  • Established protocols for successful MCMV infection in various mouse age groups.
  • Demonstrated methods for generating high-titer and virulent MCMV stocks.
  • Validated plaque assay for accurate MCMV quantification.

Conclusions:

  • The described procedures offer a robust framework for MCMV research.
  • These methods facilitate consistent and reliable studies involving MCMV in mouse models.
Keywords:
MCMVcytomegalovirus infectionsdisease modelsfibroblastsherpesviridae infectionsmicemouse cytomegalovirussalivary glandviral loadviral plaque assayvirologyvirus replication

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