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The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Published on: October 5, 2016
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Transcriptome and metabolome reveal distinct carbon allocation patterns during internode sugar accumulation in
Yin Li1, Wenqin Wang1, Yaping Feng1
1Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
Plant Biotechnology Journal
|July 28, 2018
Summary
Understanding sweet sorghum
Area of Science:
- Plant Physiology
- Biochemistry
- Genetics
Background:
- Sweet sorghum accumulates high levels of soluble sugar in its stem.
- A comprehensive understanding of carbohydrate allocation in sweet sorghum is needed.
Purpose of the Study:
- To compare the dynamic transcriptome and metabolome of sweet sorghum RIO, grain sorghum BTx406, and conversion line R9188.
- To elucidate the molecular mechanisms underlying contrasting sugar accumulation phenotypes.
Main Methods:
- Dynamic transcriptome and metabolome analysis.
- Comparative analysis of gene expression and metabolite profiles.
- Integration of transcriptomic and metabolomic data.
Main Results:
- Identified distinct sucrose metabolism features, including stable sugar phosphates in RIO and opposing trehalose-6-phosphate (T6P) trends.
- R9188 showed partial starch metabolism activity, while sweet sorghum maintained high activity in sucrose, starch, and cell wall metabolism post-anthesis.
- Differential T6P signaling in R9188, linked to BTx406 introgression, appears to down-regulate sucrose and starch metabolism.
Conclusions:
- Coordinated primary metabolic pathways drive high sucrose demand and accumulation in sweet sorghum.
- Identified key metabolic pathways and regulators for genetic improvement of carbohydrate allocation.
- Provides targets for enhancing bioenergy crop productivity.
Keywords:
RNA-seqgene expressioninternodeintrogressionmetabolomicssorghumsugar accumulationtrehalose-6-phosphate signallingMore Related Videos
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