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DoRes within CellMissy: dose-response analysis on cell migration and related data
Gwendolien Sergeant1,2, Lennart Martens1,2, Marleen Van Troys2
1VIB-UGent Center for Medical Biotechnology, Ghent, Belgium.
Bioinformatics (Oxford, England)
|July 28, 2018
Summary
We developed DoRes, a free, automated software for analyzing dose-response data in cancer research. This tool integrates with CellMissy, offering robust analysis comparable to commercial software like GraphPad Prism.
Area of Science:
- Cancer Research
- Cell Biology
- Bioinformatics
Background:
- Cell-based assays are crucial for cancer research, particularly for studying cell migration and invasion.
- Analyzing the large volumes of biological dose-response data generated presents a significant bottleneck due to the lack of automated tools.
Purpose of the Study:
- To develop an automated, free software package for dose-response analyses.
- To address the limitations in visualizing and analyzing biological dose-response data in cancer research.
Main Methods:
- Developed DoRes, an automated software package for dose-response analyses.
- Integrated DoRes as an add-on to CellMissy, an open-source tool for cell migration data management and analysis.
- Implemented non-linear curve fitting functionality within a user-friendly and flexible software package.
Main Results:
- The DoRes software package enables automated dose-response analyses.
- Demonstrated the software's capability by analyzing public dose-response data and a typical cell migration experiment.
- Showcased that extracted dose-response parameters and statistical values are comparable to GraphPad Prism.
Conclusions:
- DoRes provides a robust, user-friendly, and flexible solution for dose-response analyses in cancer research.
- The software offers a free and automated alternative to commercial tools, enhancing efficiency in biological data analysis.
- DoRes, as part of CellMissy, facilitates comprehensive management and analysis of cell migration and dose-response data.
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