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Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
Published on: February 3, 2021
A novel exonuclease-assisted isothermal nucleic acid amplification with ultrahigh specificity mediated by full-length
Xin Ye1, Yang Li2, Lijuan Wang2
1Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, P. R. China. fxech@fudan.edu.cn jlkong@fudan.edu.cn.
Abstract:
Here, we introduced a novel exonuclease-assisted isothermal nucleic acid amplification (Exo-NAT) utilizing the full-length Bst DNA polymerase combined with a melting curve analysis. This method achieved an ultrahigh specificity with a good detection limit (102 copies) in both singleplex and multiplex detection, which was validated by detecting three diarrhea-inducing pathogens, rotavirus A, astrovirus, and adenovirus, in 42 clinical samples.
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