A Simple and Effective Colorimetric Assay for Glucose Based on MnO₂ Nanosheets

Simple and effective methods for the detection of the level of blood glucose are closely linked to the monitoring of people's health. In the study, MnO₂ nanosheets with absorption range of 300 nm~500 nm and obvious yellow color were easily prepared and applied to detect glucose through their absorbance and color. The proposed method is based on the fact that a specific concentration of glucose can be quantitatively transformed into hydrogen peroxide (H₂O₂) under the catalytic effect of glucose oxidase. Based on the redox reaction of MnO₂ with H₂O₂, yellow MnO₂ can be converted into colorless Mn²⁺ to monitor the concentration of glucose. Under optimal conditions, a simple and effective visual assay for the sensitive and reliable detection of glucose was developed. The linear range was estimated to the range from 0 μM to 100 μM, with a detection limit of 12.8 μM. Furthermore, the proposed colorimetric assay based on MnO₂ nanosheets can effectively detect blood glucose of clinical serum samples with accuracy and convenience.