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Updated: Feb 7, 2026

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
Published on: March 12, 2020
Discovery of (Dihydro)pyrazine N-Oxides via Genome Mining in Pseudomonas
Ashley M Kretsch1, Gina L Morgan1, Jillian Tyrrell1
1Department of Chemistry , The University of North Carolina at Chapel Hill , 250 Bell Tower Road , Chapel Hill , North Carolina 27599 , United States.
Abstract:
Overexpression of the Pseudomonas virulence factor ( pvf) biosynthetic operon led to the identification of a family of pyrazine N-oxides (PNOs), including a novel dihydropyrazine N,N'-dioxide (dPNO) metabolite. The nonribosomal peptide synthetase responsible for production of (d)PNOs was characterized, and a biosynthetic pathway for (d)PNOs was proposed. This work highlights the unique chemistry catalyzed by pvf-encoded enzymes and sets the stage for bioactivity studies of the metabolites produced by the virulence pathway.
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