Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.9K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.9K
CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.1K
RNA Editing02:23

RNA Editing

9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
Modified Boxplots00:57

Modified Boxplots

11.2K
A standard box and whisker plot informs us about the spread of the data in a given sample. One can identify the minimum value, maximum value, first quartile value, second quartile or median value, and third quartile.
However, the box plot does not tell the reader about outliers - values that lie far from the center of the data. We can modify the standard box and whisker plot to identify the outliers and visualize the actual spread of the data in a sample.
Initially, we calculate the adjusted...
11.2K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

721
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
721

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Randomized trial of self-adaptive computerized cognitive remediation in schizophrenia.

NPJ digital medicine·2026
Same author

Caveolin-1 Modulates Islet Amyloid Polypeptide Expression Through Interaction with TXNIP in Murine Pancreatic β-Cells.

Biomedicines·2026
Same author

A Split sfGFP-Based Signal-Amplified Reporter System for Enhanced Detection of Promoter Activity.

Biotechnology journal·2026
Same author

Exploring antennal asymmetry in electrophysiological response, behavior, and olfactory gene expression in the diamondback moth, Plutella xylostella (lepidoptera: plutellidae).

Journal of insect physiology·2026
Same author

Photo-thermal crosstalk in AlGaInP and InGaN LEDs under dual-wavelength excitation.

Optics express·2026
Same author

Caveolin-1 deficiency improved glucose metabolism via modulation of β-cell autophagy in high-fat diet-fed mice.

The Journal of biological chemistry·2026
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

Related Experiment Video

Updated: Feb 7, 2026

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

4.0K

Programmable base editing in zebrafish using a modified CRISPR-Cas9 system.

Wei Qin1, Xiaochan Lu1, Shuo Lin2

  • 1Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China.

Methods (San Diego, Calif.)
|August 5, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a new base editing method in zebrafish to precisely change DNA bases, enabling better modeling of human genetic diseases caused by point mutations.

More Related Videos

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
08:00

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Published on: October 27, 2019

10.4K
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
08:31

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Published on: June 20, 2019

14.8K

Related Experiment Videos

Last Updated: Feb 7, 2026

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

4.0K
Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
08:00

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Published on: October 27, 2019

10.4K
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
08:31

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Published on: June 20, 2019

14.8K

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Zebrafish Models

Background:

  • CRISPR/Cas9 gene knockout is established in zebrafish.
  • Modeling human diseases from point mutations requires precise DNA base editing.
  • Cytidine deaminases linked to Cas9 (base editors) enable targeted single base conversions.

Purpose of the Study:

  • To establish a robust protocol for base editing in zebrafish.
  • To demonstrate the application of base editing for disease modeling.
  • To reproduce a specific mutation found in human Ablepharon-Macrostomia Syndrome.

Main Methods:

  • Utilized a Cas9-linked cytidine deaminase base editing system.
  • Applied the system to introduce specific single base mutations in zebrafish DNA.
  • Validated the efficiency and accuracy of the base editing protocol.

Main Results:

  • Successfully implemented a base editing protocol in zebrafish.
  • Demonstrated the conversion of cytidine to thymine at targeted genomic loci.
  • Reproduced a single base mutation associated with human Ablepharon-Macrostomia Syndrome.

Conclusions:

  • Base editing provides a powerful tool for precise genome engineering in zebrafish.
  • This method facilitates the creation of accurate zebrafish models for human point mutation diseases.
  • The developed protocol advances the study of genetic disorders using zebrafish models.