RpoN and the Nps and Npa two-component regulatory system control pilE transcription in commensal Neisseria
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Updated: Feb 7, 2026

Essential Components of Borreliella Borrelia burgdorferi In Vitro Transcription Assays
Published on: July 22, 2022
María A Rendón1, Beatriz Lona1, Mancheong Ma1
1The BIO5 Institute and Department of Immunobiology, University of Arizona, Tucson, Arizona.
Abstract:
Over 20 genes are involved in the biogenesis and function of the Neisseria Type IV pilus (Tfp). In the pathogenic species, RpoD and the integration host factor (IHF) protein regulate expression of pilE, encoding the Tfp structural subunit. We previously reported that in commensal species, pilE transcription is regulated by RpoN, IHF, and activator Npa. Npa has many hallmarks of response regulators in two-component regulatory systems, leading us to search for its response regulator partner. We report that Npa partners with sensor kinase Nps to control pilE transcription. Among the genes involved in Tfp biogenesis and function, only pilE is controlled by RpoN and Npa/Nps. We summarize our findings in a model, and discuss the implications of the differential regulation of pilE the context of Neisseria Tfp biogenesis.
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