Multidimensional Conductive Nanofilm-Based Flexible Aptasensor for Ultrasensitive and Selective HBsAg Detection

Area of Science:

• Nanotechnology
• Biosensing
• Biochemistry

Background:

• Hepatitis B virus (HBV) infection is a significant global health concern, leading to severe liver conditions like cirrhosis and cancer.
• Monitoring serum hepatitis B surface antigen (HBsAg) is crucial for diagnosing HBV infection.

Purpose of the Study:

• To develop a highly sensitive aptasensor for detecting HBsAg using multidimensional conductive nanofilm (MCNF)-based field-effect transistors (FET).

Main Methods:

• Fabrication of MCNF using electropolymerization of pyrrole on graphene, followed by acid treatment.
• Immobilization of HBsAg-binding aptamers onto the MCNF via covalent bonding.
• Testing the aptasensor's sensitivity and specificity against HBsAg and interfering biomolecules.

Main Results:

• The MCNF-based FET aptasensor demonstrated high sensitivity, detecting HBsAg at concentrations as low as 10 aM.
• The aptasensor showed excellent performance among various interfering biomolecules and in complex biological matrices like human serum and artificial saliva.

Conclusions:

• The developed MCNF-based aptasensor offers a promising platform for sensitive, real-time, and noninvasive diagnosis of HBV infection.
• This technology has significant potential for clinical applications in early disease detection and patient monitoring.