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Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Published on: October 15, 2021
15.2K
A tissue-based draft map of the murine MHC class I immunopeptidome
Heiko Schuster1,2,3, Wenguang Shao4, Tobias Weiss5
1Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen 72076, Germany.
Scientific Data
|August 8, 2018
Summary
This study maps the mouse MHC class I immunopeptidome across 19 tissues, identifying over 28,000 peptides. This atlas reveals tissue-specific immune responses and aids in discovering cancer antigens.
Area of Science:
- Immunology
- Proteomics
- Systems Biology
Background:
- The MHC class I immunopeptidome presents peptides to CD8+ T cells, crucial for immune surveillance.
- Knowledge of the in vivo immunopeptidome composition across tissues is limited.
Purpose of the Study:
- To create the first tissue-based atlas of the murine MHC class I immunopeptidome in healthy conditions.
- To provide data for exploring immunopeptidome tissue-specificity and identifying potential tumor antigens.
Main Methods:
- Mass spectrometry (MS) technologies were employed for peptide identification.
- Peptides were extracted from 19 normal tissues of C57BL/6 mice.
- High-confidence H2Db/Kb-associated peptides were identified and annotated.
Main Results:
- A comprehensive atlas of the murine MHC class I immunopeptidome was generated.
- A total of 28,448 high-confidence peptides were identified and annotated.
- Initial data on tissue-specificity of the immunopeptidome was established.
Conclusions:
- The developed atlas is a valuable resource for understanding tissue-specific immune responses.
- This dataset serves as a foundation for identifying tumor-associated antigens in cancer models.
- The atlas is publicly available and expected to expand for broader applications in immunology.
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