Related Experiment Video
Updated: Feb 6, 2026

09:46
Analyzing Murine Schwann Cell Development Along Growing Axons
Published on: November 21, 2012
12.1K
Mutations in dock1 disrupt early Schwann cell development.
Rebecca L Cunningham1, Amy L Herbert1, Breanne L Harty1,2
1Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
Neural Development
|August 10, 2018
Summary
Mutations in dock1 impair peripheral nervous system development in zebrafish, delaying Schwann cell myelination and axon sorting. This research highlights dock1
Area of Science:
- Neuroscience
- Cell Biology
- Genetics
Background:
- Schwann cells in the peripheral nervous system (PNS) produce myelin, essential for rapid nerve impulse transmission.
- Schwann cell maturation involves significant cytoskeletal changes, but the underlying molecular mechanisms remain unclear.
Purpose of the Study:
- To investigate the role of the atypical guanine nucleotide exchange factor, dock1, in Schwann cell development and myelination.
- To elucidate the cellular and molecular basis of myelination defects observed in dock1 mutants.
Main Methods:
- Forward genetic screen in zebrafish to identify mutations affecting myelination.
- Analysis of dock1 mutant phenotypes using whole mount in situ hybridization, transmission electron microscopy, and live imaging.
- Rescue experiments and complementation tests with engineered alleles to confirm gene function.
Main Results:
- Zebrafish dock1 mutants exhibit delayed radial sorting and reduced myelination of peripheral axons.
- Schwann cell migration and numbers are unaffected in dock1 mutants.
- Mutations in dock1 are confirmed to cause defects in PNS myelination.
Conclusions:
- Loss of dock1 function disrupts normal Schwann cell development and myelination processes.
- dock1 plays a critical role in timely radial sorting and myelination of peripheral axons in zebrafish.
Related Concept Videos
Mutations
94.5K
Overview
94.5K
Mutations
44.6K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
44.6K
Viral Mutations
39.9K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
39.9K
Mutation, Gene Flow, and Genetic Drift
64.5K
In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).
64.5K
Cancers Originate from Somatic Mutations in a Single Cell
14.9K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.9K
Point and Frameshift Mutations
1.2K
Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...
1.2K

