Related Experiment Video
Updated: Feb 6, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
Published on: November 23, 2021
Three-dimensional single-pixel imaging of incoherent light with spatiotemporally modulated illumination
Abstract:
We derive analytic expressions for the three-dimensional coherent transfer function (CTF) and coherent spread function (CSF) for coherent holographic image reconstruction by phase transfer (CHIRPT) microscopy with monochromatic and broadband illumination sources. The 3D CSF and CTF were used to simulate CHIRPT images, and the results show excellent agreement with experimental data. Finally, we show that the formalism presented here for computing the CSF/CTF pair in CHIRPT microscopy can be readily extended to other forms of single-pixel imaging, such as spatial-frequency-modulated imaging.
Related Concept Videos
Dimensional Analysis
Conversion Factors and Dimensional Analysis
The unit...
Dimensional Analysis
In fluid mechanics, dimensional...
Dimensional Analysis
Dimensional analysis allows us to analyze and compare physical quantities on a...
Light Acquisition
Light as Energy
Photons
A photon is a discrete electromagnetic particle or bundle of energy. Photons are characterized by their frequency, wavelength, and amplitude, similar to the properties of a wave. Waves with higher frequencies transmit more energy and have shorter wavelengths than longer wavelengths that transmit...
The Wave Nature of Light

