Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

14.7K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.7K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

4.5K
4.5K
Enzymes02:34

Enzymes

94.9K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
94.9K
Enzyme Kinetics01:19

Enzyme Kinetics

104.2K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
104.2K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

86.7K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
86.7K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

18.8K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
18.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel BPI3Vc-vectored chimeric BVDV antigens elicit broadly neutralizing antibodies in cattle.

Frontiers in immunology·2026
Same author

Novel BPI3Vc-vectored surface displayed fusion and hemagglutinin-neuraminidase antigens elicit broadly neutralizing antibodies in cattle.

Frontiers in immunology·2026
Same author

Live-vectored antigen cocktail confers protection against African swine fever virus (ASFV) Georgia 2007/1 challenge.

NPJ vaccines·2026
Same author

ReaxFF Parameter Set for Boron Clusters and Icosahedral Boron Crystals: Comparison with Density Functional Theory and Machine-Learning Potentials.

The journal of physical chemistry. C, Nanomaterials and interfaces·2025
Same author

Growth of Hexagonal Boron Nitride from Molten Nickel Solutions: A Reactive Molecular Dynamics Study.

ACS applied materials & interfaces·2025
Same author

A combination treatment with a water extract from Euglena gracilis and anti-PD-1 antibody strongly inhibits growth of lung cancer in mice through stimulating tumor-infiltrating lymphocytes.

International immunopharmacology·2025
Same journal

Preclinical Advances in Functionalized Nanozymes for Periodontitis: From Antibacterial Action to Tissue Regeneration.

International journal of nanomedicine·2026
Same journal

Advanced Multiscale Inhalation Platforms for Treatment of Pulmonary Diseases.

International journal of nanomedicine·2026
Same journal

Extracellular Vesicles for Therapeutic Applications: A Translational Framework Integrating Sources, Administration Routes, Indications, Quality Control, and Regulatory Systems.

International journal of nanomedicine·2026
Same journal

Exosome-Biomaterial Platforms for Diabetic Skin Infections: Microenvironment Remodeling, Responsive Delivery, and Clinical Translation.

International journal of nanomedicine·2026
Same journal

Diosmin-Capped Silver Nanoparticles Promote Osteogenic Differentiation of Human Periodontal Ligament Stem Cells: Box-Behnken Optimization and in vitro Evaluation.

International journal of nanomedicine·2026
Same journal

Surfactant-Engineered Niosomal Antibiotic Systems for Biofilm-Associated Infections: Design Principles and Translational Perspectives.

International journal of nanomedicine·2026
See all related articles

Related Experiment Video

Updated: Feb 6, 2026

Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

17.3K

Comparative functional dynamics studies on the enzyme nano-bio interface.

Spencer E Thomas1,2, Jeffrey Comer1, Min Jung Kim1

  • 1Nanotechnology Innovation Center of Kansas State, Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA, robertdelong@vet.k-state.edu.

International Journal of Nanomedicine
|August 22, 2018
PubMed
Summary
This summary is machine-generated.

Copper (Cu) and boron carbide (B4C) nanoparticles show unique effects on enzyme activity, with B4C nanoparticles completely inhibiting bioluminescence and Cu nanoparticles significantly altering enzyme function. Further research is needed to explore their potential in enzyme biochemistry.

Keywords:
2-D FDS2-dimensional fluorescence differenceAUCRLUcorrected light intensity emittedemission wavelengthexcitation wavelengthwavelength of max intensity

More Related Videos

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer
06:15

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

Published on: February 17, 2011

39.8K
Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

15.8K

Related Experiment Videos

Last Updated: Feb 6, 2026

Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

17.3K
Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer
06:15

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

Published on: February 17, 2011

39.8K
Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

15.8K

Area of Science:

  • Nanomaterials science
  • Biochemistry
  • Enzyme kinetics

Background:

  • Nanoparticles (NPs) are emerging as potential enzyme inhibitors for biomedical applications.
  • Metal oxides native to the physiological environment are of particular interest for their enzyme interactions.

Purpose of the Study:

  • To compare the functional impact of various nanoparticles (NPs) on the model enzyme luciferase.
  • To investigate the interaction dynamics between different NPs and enzyme structures.

Main Methods:

  • Utilized 2-dimensional fluorescence difference spectroscopy (2-D FDS) and 2-dimensional photoluminescence difference spectroscopy (2-D PLDS) to assess enzyme activity.
  • Employed tryptic digests and gel electrophoresis to confirm protein conformational changes.
  • Performed molecular dynamics simulations to predict NP surface modifications under physiological conditions.

Main Results:

  • Boron carbide (B4C) NPs completely ablated bioluminescence, while copper (Cu) and iron oxide (Fe2O3) NPs induced unique bimodal negative decay rates.
  • Cu NPs demonstrated a significant 271% change in enzyme activity.
  • Molecular dynamics simulations predicted NP surface capping with hydroxide or boronic acid groups, supported by zeta potential and thin layer chromatography data.

Conclusions:

  • The functional dynamics of B4C and Cu NPs on enzyme biochemistry are distinct and warrant further investigation.
  • These findings suggest potential ramifications for other enzymes, including those relevant to physiological and disease states.